Optimized Extraction Conditions and Antioxidant Activities of Ultrasonic-Extracted Antioxidant Components from Cherry Seed
Dongrui Yao, Lei Guo, Shujun Wang, Ma Rukun
Abstract
Dongrui Yao, Lei Guo, Shujun Wang, Ma Rukun
Abstract
In this study,with the DPPH radical scavenging as the index of antioxidant activity,the Box-Behnken design combined with response surface methodology(RSM) was used to optimize the ultrasonic extraction conditions of the antioxidant components from cherry seed.The optimum extraction processing parameters were determined as follows: ultrasonic power 500 W,liquid/material ratio(mL/g) 30:1,ethanol concentration 40%(V/V),extraction temperature 50℃,and extraction time 5 min.Under the optimum conditions,the total flavonoids content is(11.13 ± 0.48) mg/g of the extracts,and the extracts exhibited significant the antioxidant activities such as DPPH free radical and hydroxyl free radical scavenging effects,and reducing power.
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In this study,with the DPPH radical scavenging as the index of antioxidant activity,the Box-Behnken design combined with response surface methodology(RSM) was used to optimize the ultrasonic extraction conditions of the antioxidant components from cherry seed.The optimum extraction processing parameters were determined as follows: ultrasonic power 500 W,liquid/material ratio(mL/g) 30:1,ethanol concentration 40%(V/V),extraction temperature 50℃,and extraction time 5 min.Under the optimum conditions,the total flavonoids content is(11.13 ± 0.48) mg/g of the extracts,and the extracts exhibited significant the antioxidant activities such as DPPH free radical and hydroxyl free radical scavenging effects,and reducing power.
Key concepts: DPPH, Antioxidant, Extraction (chemistry), Response surface methodology, Chemistry, Chromatography, Ethanol, Scavenging