Inhibitory efectory of curcumin on transcriptional co-activator P300 expression of B-NHL cell line Raji cell

Aim To investigate the effect of curcumin relating transcriptional co-activator P300 on B-NHLcell line Raji,and to study anti-proliferative molecular machenism of curcumin on Raji cells.Methods Raji cells in cultured in vitro were treated by different concentrations of curcumin.The inhibitory ratio of the cells was measured by MTT assay.The expressions of P300 on Raji cells were checked by RT-PCR and Western blot.Results ①Curcumin could inhibit the growth of Raji cells and suppression was both in time-and dose-dependent manner.②Curcumin treated with Raji cells for 24 h,with the rise of concentration,can down-rogulate the expressions of both P300 mRNA and protein greatly.There were no significant difference of expressions of P300 between control and low-exposed cultures(6.25 μmol·L~(-1),P0.05),but the expression of P300 decreased at intermedieate and high concentration(12.5,25,50 μmol·L~(-1),P0.05).Conclusion Curcumin is able to inhibit the proliferation of Raji cells and dose-dependent manner,the inhibition of the Transcriptional co-activator P300 expression may be the most important mechanism.