Effect of anticancer-drugs on primary breast cancer cell kinetics and apoptesis genes bcl-2/Bax protein expression

Objective To study the effect of anticancer-drugs on primary breast cancer cell kinetics andapoptosis genes Bc1-2/Bax protein expression. Method Kinetic targets DNA index (DI) and S-phase fraction(SPF), apoptosis genes Bcl-2/Bax expression, apoptotic index (AI) and estrogen receptor (ER) were examined by using flow cytometry in the primary breast cancer patients with or without pre-chemotherapy. ResultAs compared with control group, a significant decrease in DI (P0.001), SPE (P0.001), and Baxprotein expression (P0.001) was found, but AI (0. 05P0.01) and Bcl-2 protein expression (P0. 001) displayed a significant increase in pre-chemotherapy group. No significant difference in ER was foundbetween the two groups. High percentage of AI was positively correlated with high percentage of SPF, highexpression of Bcl-2 and ER protein. But Bax showed no relation with AI. Conclusion The anticancer-drugsgave their effects by inducing cancer cell apoptosis. The above parameters can be used to judge the anticancerdrugs efficiencies and to predict prognosis.