Effect of Knocking-down Nucleostemin Gene Expression by RNA interference Technology for the U251 Cells

OBJECTIVE To investigate the expression level of nucleostemin(NS)and cell proliferation in U251 cell after interfered with RNA interference technology. METHODS Designed and synthesized siRNA aimed NS-mRNA were transfected into U251 cell lines with lipofectamineTM2000. The NS expression level was determined by RT-PCR and Western blot and cell proliferation by MTT assay. RESULTS RT-PCR and Western blot technology showed the expression level of NS-mRNA and NS-protein were 178.91% and 97.55%,respectively. The inhibition rates of NS-mRNA and NS-protein were 60.79% and 94.00% after transfected with siRNA,respectively;the MTT results showed the proliferation inhibition rate was 31.3% after interfered 72h. CONCLUSION NS gene expression was specially silenced and cell proliferation was slower after transfected with siRNA.