Alteration of Reactive Oxygen Species,NF-kB and C-IAP2 in Apoptosis of HL-60 Cell Induced by Arsenic Trioxide

BACKGROUND AND AIM: To investigate the alteration of reactive oxygen species(ROS) level,the activity of nuclear factor kappa B(NF-κB)and the expression of C-IAP2 in apoptosis of HL-60 cells induced by As2O3(arsenic trioxide,ATO).MATERIALS AND METHODS: HL-60 cells were treated with 7.5 μmol/L As2O3 alone or together with 500 μmol/L N-acetyl-L-cysteine(NAC) for 12 and 24 h.Intracellular ROS level was measured by flow cytometry(FCM),the activity of NF-κB p65 was determined by Western blot and the expression of C-IAP2 mRNA was determined by semi-quantitative RT-PCR.RESULTS: After treatment with 7.5 μmol/L As2O3 for 12 and 24 h,the level of ROS increased obviously,and the relative amount of NF-κB p65 were 49.3%±4.4% and 23.1%±2.1%,and the relative expressions of C-IAP2 mRNA were 72.9%±5.8% and 59.3%±4.4%.After co-treatment of 7.5 μmol/L As2O3 and 500 μmol/L NAC for 12 and 24 h in HL-60 cells,the level of ROS was decreased,the relative amount of NF-κB p65 were 65.4%±4.9% and 37.1%±3.4%,and the relative expressions of C-IAP2 mRNA were 81.1%±5.8% and 73.7%±4.9%.CONCLUSION: As2O3 could increase the level of ROS in HL-60,inhibited the activity of NF-κB and down-regulated the expression of C-IAP2 mRNA.Moreover,co-treatment of As2O3 and NAC could protect HL-60 cells from apoptosis through decreasing reactive oxygen species(ROS),prohibiting partly the suppression of the activity ofNF-κB and the expression of C-IAP2 mRNA.