Cloning,expression and purification of human β_2-microglobulin in pET-22b(+) vector

Objective:To construct expression vector containing β_2-M gene,further express recombinant β_2-M protein in E.coli and purify recombinant β_2-M protein.Methods:The β_2-M gene absent signal peptide was amplified by using RT-PCR.Then the β_2-M gene was cloned into pET-22b(+) vector and over expressed.The expressed protein was purified,and its activity was detected by Western blot.Results:The β_2-M protein was successfully expressed and purified.Conclusion:The over expressed recombinant β_2-M protein lays a good foundation for further constructing MHC-peptide tetramer complexes to investigate the function of CTLs.