A comparison between two methods of myelin staining in Cuprizone-induced demyelination model mice

Objective To compare two methods of myelin staining for detecting the morphological changes in the brain of Cuprizone-induced demyelination model mice.Methods Demyelination model was induced by Cuprizone which was mixed in forage and fed to mice for 4 weeks.After the brain tissue sections were made,Luxol Fast Blue(LFB) and oil red O staining methods were used to observe demyelinative changes in the corpus callosum on striatum and hippocampus sections.Results The results from both LFB and oil red O staining showed that demyelination occurred in the corpus callosum of Cuprizone-induced model mice,but the morphological changes in the striatum section were clearer detected by oil red O staining than that measured by LFB staining,and the margin of demyelination with the surrounding tissue was not clear on the section stained by LFB.Conclusion Compared with the LFB staining,Oil red O staining showed clearer demyelination and is a simple myelin staining method.