Expression of microRNA-150 in colorectal carcinoma and its clinical significance

Objective To study the expression of microRNA-150( miR-150) in colon cancer cell lines and colorectal cancer( CRC) tissues,and evaluate its clinicopathological significance. Methods miR-150 was examined in eight colon cancer cell lines,45 CRC tissues and their matched normal tissues by real-time quantitative reverse transcription PCR( qRT-PCR). The relationships between miR-150 and clinicopathological information were analyzed. Results Expression of miR-150 in eight colon cancer cell lines was lower than normal tissues. Moreover,miR-150 expression in tumor tissues was much lower than their matched normal tissues( P 0. 05). Low miR-150 level was strongly associated with the differentiated degree( P 0. 05) and pathological type( P 0. 01). Conclusion miR-150 is down-regulated not only in colon cancer cell lines but in tumor tissues,and its low expression is associated with the differentiation and pathological type. miR-150 might play a role in the course of CRC diagnosis and treatment.