Effection of Genistein on Osteoporosis Rats Induced by Estrogen Deficiency

OBJECTIVE To investigate the effect of genistein on osteoporosis in ovariectomized rats. METHODS The 23-week-old female rats were ovariectomized (the control group were shamed) to form the model rats, then the rats were divided into seven groups randomly as follows: control group, model group, GSK group(5.0 g·kg-1), nilestriol-treated group (1.5 mg·kg-1 twice per week), and genistein-treated groups at different dosage of high dose(18.0 mg·kg-1), medium dose (9.0 mg·kg-1) and low dose(4.5 mg·kg-1). The bone sample was collected and the parameters were determined after 12 weeks. RESULTS The thighbone weight, ash weight of the thigh, bone calcium, and the bone mass density of femur and lumbar were increased by genistein at dose of 4.5 and 9 mg·kg-1 after 12 week treatment (P0.05 or P0.01). The biomechanics including of maximum loading, maximum strain, flexibility loading and flexibility strain of femur were enhanced by 4.5 and 9.0 mg·kg-1 genistein (P0.05 or P0.01). The area percentage, thickness and numbers of bone trabecula were increased significantly and saeptum of bone trabecula was decreased in the groups treated by 4.5 and 9 mg·kg-1 genistein (P0.05 or P0.01). The level of ALP in serum and the level of Ca/Cr and Pyd/Cr in urine were decreased in the groups administered with genistein at dose of 4.5 and 9 mg·kg-1 (P0.05 or P0.01). CONCLUSION Genistein could improve the bone mass density, biomechanics and correlated biochemical indicators ovariectomized rats and could be used for osteoporosis treatment.