2000•Xi'nan nongye xuebaoRequires access

Efficient transformation of recombinant plasmid DNA by electroporation into Agrobacterium tumefaciens

Bo Yan

Abstract

Efficient transformation of Agrobacterium tumefaciens LBA4404 by electroporation was performed using the binary vector PAI121 that contain α-PAP gene and 35s promoter. The optimal electroporation condition for plasmid transfer was obtained at 18kv/cm, 25μF and 200Ω. And the growth stage of A. tumefaciens was OD600= 0. 4 --0. 5. The frequency of transformation was a linear function of the DNA concentration.

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Key concepts: Electroporation, Agrobacterium tumefaciens, Transformation (genetics), Plasmid, Transformation efficiency, Biology, Agrobacterium, Recombinant DNA