Effect of different concentrations of blood glucose on expression of Bcl-2 gene in brain of neonatal rats with hyoxia-ischemia

Objective To investigate effect of different concentrations of blood glucose on expression of Bcl-2 gene, using competitive RT-PCR. Methods The newborn SD rats aged 7days was divided into eight groups according to times when HI occured. After establishment of eight HI models (including normal control group and false operation group), the rats were killed and their brain tissues sample were taken. Changes in expression of Bcl-2 in brain tissues in each model group were observed and compared to evaluate objectively cerebral protection of glucose for rate with HI. Results The results showed that expression time of bcl-2 gene delayed at least for 12 hours, and in the group with higher hyperglycemia before HI, expression intensity of the gene was stronger than that in HI group. However, expression of the gene in the group with hypoglycemia before HI was significantly weaker than that in HI group. Conclusion It was suggested that cerebral damage effect of hypoglycemia before HI can be probably induced through downregulation of the gene partially, and cerebral protective effect of hyperglycemia before HI can be probably induced through upregulation of the gene partially.