2005Chinese Bulletin of BotanyRequires access

Research Progress in Plant Cytoplasmic Male Sterility by Protoplast Fusion

Chun Fu

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Abstract

Protoplast fusion for combining and exchanging nuclear and cytoplasm genomes in hybrid progeny provides a new strategy to improve traits controlled by chloroplast or mitochon- drial DNA. This paper reviews the methods and mechanisms of obtaining cybrids, advances in transforming cytoplasmic male sterility (CMS), the potential use of these CMS somatic hybrids and the genetic transformation of CMS in sex hybrids. In addition, the application of this strat- egy to breeding seedless woody fruit trees is discussed.

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Protoplast fusion for combining and exchanging nuclear and cytoplasm genomes in hybrid progeny provides a new strategy to improve traits controlled by chloroplast or mitochon- drial DNA. This paper reviews the methods and mechanisms of obtaining cybrids, advances in transforming cytoplasmic male sterility (CMS), the potential use of these CMS somatic hybrids and the genetic transformation of CMS in sex hybrids. In addition, the application of this strat- egy to breeding seedless woody fruit trees is discussed.

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Available abstract

Protoplast fusion for combining and exchanging nuclear and cytoplasm genomes in hybrid progeny provides a new strategy to improve traits controlled by chloroplast or mitochon- drial DNA. This paper reviews the methods and mechanisms of obtaining cybrids, advances in transforming cytoplasmic male sterility (CMS), the potential use of these CMS somatic hybrids and the genetic transformation of CMS in sex hybrids. In addition, the application of this strat- egy to breeding seedless woody fruit trees is discussed.

Key concepts: Cytoplasmic male sterility, Protoplast, Sterility, Biology, Hybrid, Cytoplasm, Somatic cell, Somatic fusion

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