Effect of ionizing radiation on cell cycle progression of Jurkat T cells

Objective To investigate the change regularity of cell cycle progression in Jurkat T cells damaged by ionizing radiation.Methods The time-and dose-effects of cell cycle progression in Jurkat T cells after X-irradiation with different doses(0.075,0.5,1.0,2.0,4.0 and 6.0 Gy) were detected with flow cytometry.Results For the time-effect,the percentage of Jurkat T cells in S phase immediately increased after irradiation with 2.0 Gy X-rays,and that of G2 + M phase cells increased significantly 8 h after irradiation as compared with that in the sham-irradiation(P0.001).For the dose-effect,the percentage of G0/G1 phase cells 18 h after irradiation with 0.075 Gy and 0.5-6.0 Gy was significantly lower than that in the sham-irradiation in a dose-dependent pattern(P0.001),while the percentages of S and G2 + M phase cells increased significantly(P0.001).Conclusion Ionizing radiation with 0.5-6.0 Gy X-rays could induce S delay and G2 arrest in Jurkat T cells in a dose-dependent pattern.