Expression of HPV16,18E6 and MCM7 proteins in mammary invasive duct carcinomas

Objective:To examine the expression of HPV16,18E6 and MCM7 proteins in mammary invasive duct carcinomas and in benign breast diseases and explore its relation between high righ HPV infection with disorder of cell cycle replication regulatory function and the carcinogenesis and progression of human breast cancer.Methods:SP immunohistochemical teachnique was used to detect the expression of HPV16,18E6 and MCM7 in 52 cases of mammary invasive duct carcinomas,30 cases of breast adenosis and 30 cases of normal breast tissues.Results:Positive rates of HPV16,18E6 and MCM7 protein were significantly higher in breast cancer than that in normal breast and breast adenosis (P0.02,P0.05).Positive expression of HPV16,18E6 was positively correlated with expression of MCM7 (r=0.5442,P0.001).MCM7 protein expression was associated with histological grading(P0.01),axillary lymph node metastasis(P0.03) and tumor size(P0.01)in invasive ductal carcinoma group.Conclusion:HPV16,18 type infection and high expression of MCM7 protein in human mammary epithelial cell with cell cycle replication regulatory disorder may play an important role in the pathogenesis and development of human breast cancer.The high expression of MCM7 was associated with cell proliferation,invasiveness and metastasis of invasive ductal carcinoma.The combined detection of HPV16,18E6 and MCM7 protein may be useful predictors of cell proliferation,biological behavior and prognosis of breast cancer.