2008•Journal of Hebei Normal UniversityRequires access

Simple and Rapid Method of Extracting and Purifying DNA from Soil

HE Jian-gong

Abstract

Different methods were studied and compared to extract and purify DNA from the soil,high purity and quality DNA can be yielded by the TENS-ammonium acetate method containing freeze-thaw cycles,bead-beating procedure.The size of DNA was above 23.1 kb with no obvious degradation.The PVPP spin column chromatography method shows high yield and quality than the Agarose Gel DNA Purification Kit.

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Key concepts: Chromatography, DNA, Agarose, Ammonium acetate, Yield (engineering), DNA extraction, Degradation (telecommunications), Chemistry