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2011•Chinese Journal of Laboratory DiagnosisRequires access

Diosgenin-induced apoptosis dependent on caspase-3 pathway in MGC-803 cells

Wang Tie-chen

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Abstract

Objective AIM To study the antitumor mechanism of Dio for human gastric adenocarcinoma MGC-803 cells.Methods MGC-803 cells viability and the effect of caspase-3 inhibitor(DEVD-CHO) on Dio-induced apoptosis were measured by MTT assay.Annexin V expression in MGC-803 cell membrane was assessed by a flow cytometer.The effect of Dio on DNA in MGC-803 cells was measured using APO-BRDU Kit.Caspase-3 activity was detected by a enzyme link immune detector.Results Dio inhibited MGC-803 cells growth in dose-and time-dependent manners.MGC-803 cells treated with Dio showed typical characteristics of apoptosis:Annexin V-FITC+/PI-and DNA fragmentation.Caspase-3 in MGC-803 cells was activated by Dio.Caspase-3 inhibitor,Ac-DEVD-CHO partially inhibited Dio-induced apoptosis.Conclusion Dio-induced apoptosis is partially dependent on caspase-3 pathway in MGC-803 cells.

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What this paper is about

Objective AIM To study the antitumor mechanism of Dio for human gastric adenocarcinoma MGC-803 cells.Methods MGC-803 cells viability and the effect of caspase-3 inhibitor(DEVD-CHO) on Dio-induced apoptosis were measured by MTT assay.Annexin V expression in MGC-803 cell membrane was assessed by a flow cytometer.The effect of Dio on DNA in MGC-803 cells was measured using APO-BRDU Kit.Caspase-3 activity was detected by a enzyme link immune detector.Results Dio inhibited MGC-803 cells growth in dose-and time-dependent manners.MGC-803 cells treated with Dio showed typical characteristics of apoptosis:Annexin V-FITC+/PI-and DNA fragmentation.Caspase-3 in MGC-803 cells was activated by Dio.Caspase-3 inhibitor,Ac-DEVD-CHO partially inhibited Dio-induced apoptosis.Conclusion Dio-induced apoptosis is partially dependent on caspase-3 pathway in MGC-803 cells.

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Available abstract

Objective AIM To study the antitumor mechanism of Dio for human gastric adenocarcinoma MGC-803 cells.Methods MGC-803 cells viability and the effect of caspase-3 inhibitor(DEVD-CHO) on Dio-induced apoptosis were measured by MTT assay.Annexin V expression in MGC-803 cell membrane was assessed by a flow cytometer.The effect of Dio on DNA in MGC-803 cells was measured using APO-BRDU Kit.Caspase-3 activity was detected by a enzyme link immune detector.Results Dio inhibited MGC-803 cells growth in dose-and time-dependent manners.MGC-803 cells treated with Dio showed typical characteristics of apoptosis:Annexin V-FITC+/PI-and DNA fragmentation.Caspase-3 in MGC-803 cells was activated by Dio.Caspase-3 inhibitor,Ac-DEVD-CHO partially inhibited Dio-induced apoptosis.Conclusion Dio-induced apoptosis is partially dependent on caspase-3 pathway in MGC-803 cells.

Key concepts: Apoptosis, Annexin, DNA fragmentation, Chemistry, Molecular biology, Flow cytometry, Caspase, Caspase 3

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