2004•Chinese Journal of Clinical NeurosciencesRequires access
Impact of Focal Cerebral Ischemia/Reperfusion on Circulating CD34~+ Cells in Rat
Xiang Hu
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Abstract
Aim:This study was intended to detect changes of circulating CD34 + cells after focal cerebral ischemia/reperfusion in rats.Methods:A model of a suture occlusion of right middle cerebral artery (MCA) of male SD ratd was used.60 mature male SD rats were randomly divided into 12 groups:control group,sham-operated control group,1-hour (1h),3h,6h,12h,24h,2-day (2d),3d,4d,7d,,14d reperfusion groups.After right middle cerebral artery occlude,the rats were suffered from ischemia for 2 hours, then reperfusion was allowed for different time as mentioned above before decapitation.Circulating CD34 + cells were detected by cytoflowmetric analysis.Results:There was no influence on circulating CD34 + cells in rat MCAO models from 1h to 2 days after reperfusion.But circulating CD34 + cells sharply decreased from 3 to 7 days after reperfusion and returned to basal values at 14th day after cerebral reperfusion.Conclusion:Circulating CD34 + cells sharply decreased from 3 to 7 days after reperfusion in rat MCAO models.