Effects of culture conditions on sporulation of Alternaria solani

The sporulation ability of Alternaria solani is very limited when it is grown in vitro. The current paper deals with the effects of media, mycelial wound, ultraviolet light treatment, and temperature alteration on conidial production of 7 strains. It is found that all these treatments have significant effects on sporulation. The optimum condition for large amount sporulation of A. solani was determined. Totally 160 strains of A. solani isolated from various locations were investigated for sporulation by using optimizing sporulation conditions in vitro. The results showed that 120 strains(75% of the total) could produce a large number of conidia, ranged from 1.26×104conidia/cm2 to 5.0×104conidia/cm2.