Forward-reverse Evaluation on Bio-active Contribution of Main Herb Pairs Including 'Rhizoma Cyperi-Rhizoma Corydalis' and 'Rhizoma Cyperi-Angelicae Sinensis Radix' in Xiangfu Siwu Decoction

Objective:The bio-active contribution of main herb pairs in Xiangfu Siwu decoction(XFSWD) was evaluated by using the forward-reverse biological activity test.Method: Four animal models,including hot-plate test,acetic acid-induced writhing,dysmenorrheal mice model,and blood-deficiency mice model,were adopted to test the effect of every sample on different models.Result: In the three pain models,Rhizoma Cyperi-Rhizoma Corydalis(RCRC)have great analgesic effect.When RCRC are removed from XFSWD,its analgesic activities was lowered,even disappeared.While the analgesic effect of Rhizoma Cyperi-Angelicae Sinensis Radix(RCASR) was weaken than RCRC;and when RCASR was removed from XFSWD,the effect was not diminished clearly,some were even increased.In comparison with the model group,the amount of white blood cells,the weight of spleen and thymus of all samples had no significant difference in the blood deficiency experiment.Based on the number variation of red blood cells and hemoglobin,RCASR had strong blood-enriching function,while XFSWD and XFSWD removed RCASR showed no apparent effect.Further research showed that XFSWD could raise NO level and SOD activity,degrade Ca2+ level in the uterus of dysmenorrheal mice.Conclusion: In XFSWD,the blood-enriching effect of RCASR is very weak,RCRC plays important role in its analgesia effect.The inhibiting dysmenorrhea of XFSWD may be resulted from affecting the level of NO and Ca2+,and SOD activity.The forward-reverse bio-activity evaluation is an effectual method for studying on the compatibility regularity of traditional Chinese medicine formulae.