Inhibition of the proliferation of HT-29 and the expression of survivin in HT-29 cells by Vpr

Objective To investigate the effect of Vpr on proliferation of HT-29 and expression of Survivin gene.Methods HT-29 cells were treated with Ad-Vpr or Adv for five days,The cell proliferation was measured by MTT assay everyday.72h post treatment,Flow cytometry(FCM) was employed to detect the cell cycle distribution and apoptosis.The expression of Vpr protein was detected by western blot;The rnRNA and protein expression levels of Survivin was determined by RT-PCR or Western blot.Results MTT showed that Vpr significantly inhibited the proliferation of HT-29 cells(72h post treatment,MOI=200 P0.05 vs.control or Adv group) in a time dependent manner.The results of FCM showed that Vpr markedly increased the ratio of apoptosis(72 h post treatment,MOI=200,P0.01 vs.control or Adv group) and cells in G2/M cell cycle(72 h post treatment,MOI=200,P0.05 vs.control or Adv group).RT-PCR and Western blot showed that Vpr significant1ly downregulated the expression levels of Survivin(72 h post treatment,MOI:50,P0.05 or P0.01 vs.control or Adv(MOI=200) group).Conclusion Vpr inhibits the proliferation of HT-29 in vitro by inducing cell cycle arrest and apoptosis.and Vpr significant1ly downregulated the expression of survivin.Vpr is a potential anticancer agent for colorectal cancer.