In vitro antioxidant activity of Borojoa sorbilis Cuter.

Objective To study in vitro antioxidant effects of Borojoa sorbilis Cuter.Method Concentrated solution of fresh fruits of B.sorbilis Cuter.as well as fractions which eluted through macroporous resin column,were taken as total sample,unabsorbed part,water part,30% alcohol part and 60% alcohol part.Five samples were measured by three different in vitro tests:2,2-diphenyl-1-picrylhydrazyl(DPPH) radical scavenging activity,2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid(ABTS) radical scavenging activity and ferric reducing antioxidant power(FRAP).Ascorbic acid(Vc) was used as a positive control.The possible material basis of antioxidant activities was analyzed by LC-MS.Results Samples showed good DPPH radical scavenging effect,while ABTS radical scavenging effect and ferric reducing antioxidant power activity are not obvious.Ion peak clusters in the total ion chromatogram(TIC),the retention time at 4.00-5.00 min,may be involved in antioxidant activity.Conclusion B.sorbilis Cuter.has moderate antioxidant activity compared with Vc,and its material basis may be related to the notable ion peaks in TIC.