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2002•Zhongguo weishengtaixue zazhiRequires access

AN IMPROVED METHOD OF COUNTING BIFIDOBACTERIUM IN STOOL

Yong Li

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Abstract

Objective:To prepare a medium for identifying and counting Bifidobacterium in stool.Methods:Majority of Bifidobacterium have Tilactase and upper enzyme activity,so add X Gal in the improved self made BS medium as the substrate,Bifidobacterium hydrolysis substrate and release indole,which induces color response.Results: Bifidobacterium colony show dark blue,and other Bacteria generally show white or light blue.Conclusion:For the obvious identification between and other Bacteria on the improved self made medium,it can apply to identify and count Bifidobacterium in stool.

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What this paper is about

Objective:To prepare a medium for identifying and counting Bifidobacterium in stool.Methods:Majority of Bifidobacterium have Tilactase and upper enzyme activity,so add X Gal in the improved self made BS medium as the substrate,Bifidobacterium hydrolysis substrate and release indole,which induces color response.Results: Bifidobacterium colony show dark blue,and other Bacteria generally show white or light blue.Conclusion:For the obvious identification between and other Bacteria on the improved self made medium,it can apply to identify and count Bifidobacterium in stool.

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Available abstract

Objective:To prepare a medium for identifying and counting Bifidobacterium in stool.Methods:Majority of Bifidobacterium have Tilactase and upper enzyme activity,so add X Gal in the improved self made BS medium as the substrate,Bifidobacterium hydrolysis substrate and release indole,which induces color response.Results: Bifidobacterium colony show dark blue,and other Bacteria generally show white or light blue.Conclusion:For the obvious identification between and other Bacteria on the improved self made medium,it can apply to identify and count Bifidobacterium in stool.

Key concepts: Bifidobacterium, Actinomycetaceae, Bacteria, Microbiology, Substrate (aquarium), Feces, Chemistry, Bifidobacterium breve

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