Influence of a Combination of Mild Hypothermia and Sevoflurane on Oxygen-Glucose Deprivation Injury in Rat Hippocampal Slices

Objective:To explore the role of amino acids in the extracellular fluid of rat's hippocampus slices in the protective effects of combination of mild hypothermia and sevoflurane on oxygen-glucose deprivation injury through HPLC.Methods: Hippocampal slices were randomly divided into 4 groups(n=8 each): GroupⅠOGD;Group Ⅱsevoflurane(Sev);Group Ⅲ mild hypothermia(MH) and Group ⅣMH+ Sev oflurane.Slices were randomly exposed to experiment condition for 14 min before OGD and for another 14 min during OGD.The concentration of amino acid aspartic acid(Asp),glutamic acid(Glu),glycine(Gly),taurine(Tau),γ-aminobutyric acid(GABA) before OGD,at the end of OGD and 30 min,60 min of reoxygenation were detected.Results: Compared with the OGD group,the concentration of Asp、Glu and Gly decreased,but Tau and GABA increased in other groups at the end of OGD and 30 min,60min of reoxygenation.In the group of MH+Sev,the levels of Asp and Glu decreased but the levels of Tau and GABA increased;compared with the other groups.Conclusions: The protective effect of combination of mild hypothermia and sevoflurane on OGD injury in rat hippocampal slices is associated with the decrease in the level of extracellular excitatory amino acid.