A novel method for rapid identification and drug resistance of Moraxella catarrhalis

Objective To establish a novel method for rapid identification and drug resistance of Moraxella catarrhalis, Different sourses of blood and the formula of chocklate agars were detected the Growth Index(GI). Methods Moraxella catarrhalis from specimens contaminated by microbial flora were isolated with a choclate agar with 50 mg/L vancomycin (CHOC-V) method. Nine media were inoculated,on which GI were calculated and compared. beta-Lactamase were determined by nitrocefin slip method. Results 228 strains of Moraxella catarrhalis and 203 strains of recent limbus bacteria were examined. The sensitivity of this method was 100%,and the specificity was 100%. The GI of Moraxella catarrhalis on chocklate agars and blood agars was higher than that of the BCA, NUA, MHA ( P0.05). beta-Lactamase positive rate in Moraxella catarrhalis was 93.0%. The MIC 50 and MIC 90 of PIPC/SBT and PIPC/TAZ against Moraxella catarrhalis were 8 times lower then PIPC.The MIC 50 and MIC 90 of AMP/SBT against Moraxella catarrhalis were 8 times lower then AMP. Conclusions This method is rapid, accurate and simple for identifying Moraxella catarrhalis. PIPC/SBT,CFP/SBT,PIPC/TAZ,AMP/SBT,IPM/CST showed high activity against Moraxella catarrhalis of beta-Lactamase positive.