INFLUENCE OF DIFFERENT COLD ISCHEMIA TIME ON SURVIVAL RATE FOLLOWING PARTIAL LIVER TRANSPLANTATION IN THE RATS

Objective To evaluate survival rate of different cold preservation time in a novel model of partial liver transplantation in rats.Methods Wister rats subjected to 50% liver resection (control:group A) were compared with rats 50% partial liver transplantation with different cold ischemia.Livers were preserved at 4 degrees C for 45 minutes(group B),4 hours (group C)and 10 hours (group D) in University of Wisconsin solution.The expression of the proliferative cell nuclear antigen (PCNA) of liver in rats were evaluated by immune histochemical method 1d,2d,4d after operation,repectively.Result The survival rate with group B,group C.group D was 80%,45%,25%,and the expression of PCNA peak was 54±5.2;44±3.0;20±1.9 at 48h after transplantation,respectively.Prolonged time of cold preservation(10 hours)was associated with a dramatic decrease of PCNA ( P 0.05).Conclusion The expression of PCNA peak in partial liver transplantation slightly delayed compared with hepatectomy.Prologed cold preservation significantly decreased the regenerative ability of the liver and animal survival in partial liver transplantation in the rats.