The Expression of Caspase-9 in Rabbit Retinal Ischemia-Reperfusion and Estrogen Influence

Objective:To investigate the retinal ischemia-reperfusion and the effects of Caspase-9expression and effect of estradiol benzoate.Methods:The animals were randomly divided into sham group,ischemia group,estrogen treatment group,ischemia group and treatment group was divided into the the reperfusion after 1h,6h,12h,24h,48hand 72h6period.Production retinal ischemia-reperfusion injury in animal models,retinal morphological changes were observed,different times of the retinal tissue of Caspase-9expression detected by immunohistochemistry SABC method.Results:Estrogen therapy group observed indicators expression.Results compared with ischemia group,relative expression significantly reduced reperfusion 6h,12h,24h,48h,72hdifference P0.05,statistically significant.Conclusion:Estradiol benzoate can inhibit retinal ischemia reperfusion injury apoptosis gene Caspase-9in increased retinal expression,and inhibition of apoptosis to achieve its therapeutic effect on retinal ischemia reperfusion injury.