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Construction of plant expression vector containing Arabidopsis transcription factor CBF4

Haixia Zhao

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Abstract

CBF4 gene was obtained by enzyme cutting with both SalI and EcoRI,and inserted into Bluescript M13(SK),thus the intermediate vector SK-CBF4 was formed.Then,SK-CBF4 and HpBPC26-CBF4 were double digestion using BamHI and KpnI,respectively,and connected them using T4DNA.Finally,the plant expression vector of HpBPC-CBF4 has been constructed.The results of PCR and enzyme digestion showed the plant expression vectors were correct and can be use in the genetic transformation of monocotyledon.

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What this paper is about

CBF4 gene was obtained by enzyme cutting with both SalI and EcoRI,and inserted into Bluescript M13(SK),thus the intermediate vector SK-CBF4 was formed.Then,SK-CBF4 and HpBPC26-CBF4 were double digestion using BamHI and KpnI,respectively,and connected them using T4DNA.Finally,the plant expression vector of HpBPC-CBF4 has been constructed.The results of PCR and enzyme digestion showed the plant expression vectors were correct and can be use in the genetic transformation of monocotyledon.

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Available abstract

CBF4 gene was obtained by enzyme cutting with both SalI and EcoRI,and inserted into Bluescript M13(SK),thus the intermediate vector SK-CBF4 was formed.Then,SK-CBF4 and HpBPC26-CBF4 were double digestion using BamHI and KpnI,respectively,and connected them using T4DNA.Finally,the plant expression vector of HpBPC-CBF4 has been constructed.The results of PCR and enzyme digestion showed the plant expression vectors were correct and can be use in the genetic transformation of monocotyledon.

Key concepts: EcoRI, Expression vector, Transformation (genetics), Arabidopsis, Vector (molecular biology), BamHI, Restriction enzyme, Biology

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