2012•Zhonghua laonian xin-nao-xueguanbing zazhiRequires access
Role of ATP postconditioning in protection of rabbits against myocardial I/R injury
LI Nin-nin
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Abstract
Objective To study the role of ATP postconditioning in protection of rabbits against myocardial I/R injury and its mechanism by observing its effect on expression of protein kinase P(Akt)and ERK1/2.Methods Forty-eight male New Zealand white rabbits were randomly divided into I/R group,ATP postconditioning group,wortmannin+ ATP postconditioning group and PD98059 + ATP postconditioning group(12 in each group).An acute myocardial I/R model was established.Myocardial inarction size and apoptosis index were assayed at the end of experiement. Expressions of myocardial p-Akt and p-ERK1/2 were detected by Western blot.Results The infarction size and apoptosis index were significantly lower in ATP postconditioning group than in I/R group(P0.01).Western blot showed that the expression levels of p-Akt and p-ERK1/2 were significantly higher in ATP postconditioning group than in I/R group(P0.05).Conclusion ATP postconditioning can reduce the myocardial infarction size and apoptosis index and up-regulate the expression of p-Akt and p-ERK1/2 in rabbits following I/R injury,indicating that the PI3K/Akt and ERK1/2 signal patheways participate in protecting rabbits against myocardial I/R injury.