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2015•Xiandai shengwu yixue jinzhanRequires access

Effects of Calcification on Rat Aortic Induced by Warfarin

Jiang Xiao-y

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Abstract

Objective:To study the effect of calcification on rat aortic induced by warfarin(WFN).Methods:The aortic calcification was induced by WFN(3.0 mg/g) in rats.Rats were divided into three groups randomly:Control group,6 W Calcification group and 12 W Calcification group.O-cresol peptide complex ketone colorimetric method and Von Kossa staining was used to detect the calcification formation.Using doable immunofluorescent labeling with TUNEL and DAPI,Vascular Smooth Muscle Cell(VSMC)apoptosis in rats were observed with a laser confocal microscope.The expression levels of Gas 6 in VSMC was determined by Western blot.Results:The calcium nodule,calcium deposition and VSMC apoptosis in calcification group were higher than Control group,but the level of Gas 6 protein expression was decreasing.The apoptosis and calcification has significant correlation(R2= 0.8853,P 0.0001).Conclusion:WFN treatment may induce aortic calcification via decreasing the level of Gas 6 protein in experimental rats.

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Objective:To study the effect of calcification on rat aortic induced by warfarin(WFN).Methods:The aortic calcification was induced by WFN(3.0 mg/g) in rats.Rats were divided into three groups randomly:Control group,6 W Calcification group and 12 W Calcification group.O-cresol peptide complex ketone colorimetric method and Von Kossa staining was used to detect the calcification formation.Using doable immunofluorescent labeling with TUNEL and DAPI,Vascular Smooth Muscle Cell(VSMC)apoptosis in rats were observed with a laser confocal microscope.The expression levels of Gas 6 in VSMC was determined by Western blot.Results:The calcium nodule,calcium deposition and VSMC apoptosis in calcification group were higher than Control group,but the level of Gas 6 protein expression was decreasing.The apoptosis and calcification has significant correlation(R2= 0.8853,P 0.0001).Conclusion:WFN treatment may induce aortic calcification via decreasing the level of Gas 6 protein in experimental rats.

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Available abstract

Objective:To study the effect of calcification on rat aortic induced by warfarin(WFN).Methods:The aortic calcification was induced by WFN(3.0 mg/g) in rats.Rats were divided into three groups randomly:Control group,6 W Calcification group and 12 W Calcification group.O-cresol peptide complex ketone colorimetric method and Von Kossa staining was used to detect the calcification formation.Using doable immunofluorescent labeling with TUNEL and DAPI,Vascular Smooth Muscle Cell(VSMC)apoptosis in rats were observed with a laser confocal microscope.The expression levels of Gas 6 in VSMC was determined by Western blot.Results:The calcium nodule,calcium deposition and VSMC apoptosis in calcification group were higher than Control group,but the level of Gas 6 protein expression was decreasing.The apoptosis and calcification has significant correlation(R2= 0.8853,P 0.0001).Conclusion:WFN treatment may induce aortic calcification via decreasing the level of Gas 6 protein in experimental rats.

Key concepts: Calcification, Von Kossa stain, Apoptosis, TUNEL assay, Western blot, Vascular smooth muscle, Chemistry, Calcium

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