2014•Journal of Nanchang UniversityRequires access

Dual-Replicon Plasmids for Elimination of Resistant Plasmids

Mo Bin

Abstract

Objective To observe the eliminating effect of dual-replicon plasmids on resistant plasmids.Methods The dual-replicon plasmids pKT230-oriV were constructed and transferred into bacteria with resistant plasmids pRK290through the transformation and conjugational transfer pathways.The eliminating effect of dual-replicon plasmids on resistant plasmids was observed.Results The bacteria were cultured for 5generations after the transfer of dual-replicon plasmids,and resistant plasmids pRK290in bacteria were eliminated.Conclusion The conjugational transfer of dual-replicon plasmids is a pathway to eliminate resistant plasmids.

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Key concepts: Replicon, Plasmid, T-DNA Binary system, Bacteria, Biology, Transformation (genetics), Microbiology, Genetics