2009•Heilongjiang nongye kexueRequires access
Construction of T-Vector
Liang Chen
Open publisher page 3 citations
Abstract
A method for construction of a cloning vector(T-vector) for direct ligation with PCR products was described in this passage.The T-vector derived from pcDNA3.1/V5-His was the skeleton vector which was a linear vector derived by restriction enzyme EcoR V and obtained a single T with 3' overhanging by dTTP and Taq polymerase.After the ligation with destination gene and T-vector,the transformation of E.coli,plasmids extracted were confirmed by restriction endonuclease digestion,the results showed that the constructed T-vector successfully cloned the destination gene fragment,and the construction of T-vector was successful.