Effect of Lactuside B on the Expression of CHOP mRNA in Rats′ Cerebral Cortex and Hippocampus after Cerebral Ischemia-Reperfusion Injury

OBJECTIVE To investigate the effect of lactuside B on CHOP mRNA expression in rat's cerebral cortex and hippocampus after cerebral ischemia injury.METHODS Male SD rats of 280-320 g were randomly given brain ischemia-reperfusion treatment for 24 and 72 h respectively and then divided into sham operation group,model group,positive control group,and lactuside B 12.5 mg·kg-1 group,25 mg·kg-1group and 50 mg·kg-1 group.There were a total of 12 groups with 4 rats in each group.The cerebral ischemia-reperfusion model was established by cerebral artery occlusion for 2 h.The animals with neurological missing symptom scores of 1-3 were selected for the experiment.All the animals were intraperitoneally injected corresponding experimental drugs after reperfusion for 24 and 72 h and then sacrificed.RT-PCR was used to detected the expression of CHOP mRNA in the cerebral and hippocampus at different time.RESULTS After cerebral ischemia-reperfusion injury for 24 and 72 h,the neurological deficits scores of the model group animals were significantly higher compared with the sham group(P0.01);the neurological deficit scores of the animals were decreased for positive control group and all lactuside B groups compared with model group(P0.01).All of the three dosage(12.5,25 and 50 mg·kg-1)of lactuside B could reduce CHOP mRNA expression in cerebral cortex and hippocampus compared with the model group(P0.05,P0.01),and the majority of lactuside B groups were superior to the positive control drug nimodipine(P0.01).There was a good dose-effect relationship for lactuside B in general,but only the middle dose group showed good time-effect relationship.CONCLUSION The results suggest that the anti-cerebral ischemia effect of lactuside B may be related with the down-regulation of CHOP mRNA expression in cortex and hippocampus.Lactuside B is worthy of development.