Acceleration of Apoptosis by Transfection of Apoptin Gene in Multi-drug Resistant Bladder Cancer Cells

Objective To observe the effects of Apoptin gene on killing MDR bladder cancer cells and illustrates its mechanisms.Methods Apoptin gene was transfected into MDR bladder cancer cells by liposome.The mRNA of Apoptin and bcl-2 were detected by RT-PCR.The protein of Apoptin and p53 were detected by SABC immunohistochemistry.The growth rate of human bladder cancer cells were studied by constructing the growth curve and TUNEL,cell apoptosis was observed by flow cytometry.Results The expression of Apoptin mRNA was positive in EJ/Apoptin cells(P0.05).Apoptin protein expression of EJ/Apoptin cells was positive and p53 protein expression was not increased(P0.05).The growth of MDR bladder cancer cells was significantly inhibited after bak gene was transfected(P0.05).Apoptosis of cells increased significantly.The apoptosis rate was 35%.Conclusion Apoptin gene could inhibit the growth of MDR bladder cancer cells effectively.Inducing cell apoptosis by up-regulating the expression of p53 gene might not be one of its mechanisms.