Chemical constituents of Illicium brevistylum

Objective To isolate and identify the chemical constituents in the ethanol extract from the barks of Illicium brevistylum.Methods The barks of I.brevistylum were extracted by 80% ethanol then partitioned by system solvents with different polarity.The ethyl acetate extract was separated on silica gel,Sephadex LH-20,and ODS columns.The isolated compounds were identified by physicochemical properties and spectral analyses.Results Twelve compounds were isolated and purified from the ethyl acetate extract of I.brevistylum and the structures were identified as quercetin 3-O-β-xylopyranoside(1),quercetin(2),dihydro kaempferol(3),kaempferol(4),wulingzhic acid V(5),catechin(6),3,5,7-trihydroxylchromone-3-O-α-L-arabinopyranoside(7),majucin(8),anislactone B(9),7-O-acetylanislactone B(10),dunnianolide C(11),and taxifolin-3-O-β-xylopyranoside(12).Conclusion Compounds 5 and 7 are obtained from the plants in Illiciaceae for the first time and compounds 1,6,and 8—11 are firstly isolated from I.brevistylum.