Expression of transforming growth factor-β_1、tumor necrosis factor-α and interventional mechanism of Chinese medicinal with actions of replenishing qi and reinforce the healthy qi in rat spleen-deficiency model

Objective:To study the changes of expressions of transforming growth factor-β1、tumor necrosis factor-α in spleen-deficiency rat model,and the interventional effects of Chinese medicinal with actions of replenishing qi and reinforce the healthy qi.Methods:All rats(n=40) were randomly divided into the normal group,model group,treatment group 1(T group 1,treated with Sijunzi Decoction) and treatment group 2(T group 2,treated with Yupingfeng Powder,eachn=10).The spleen-deficiency model was established in rats by using the methods of purgation,improper diet and over strain.The expressions of TGF-β1、TNF-α in the ventral nucleus of hypothalamus and CA1 zone of hippocampus,and therapeutic effects of Sijunzi Decoction and Yupingfeng Powder were detected by applying immunohistochemistrical assay and in situ hybridization.Results:In the model group the TGF-β1、TNF-αexpression decreased significantly;Compared with model group,TGF-β1、TNF-αexpressions of treatment group1 and group 2 increased significantly in the ventral nucleus of hypothalamus and CA1 zone of hippocampus.Conclusion:Sijunzi Decoction and Yupingfeng Powder,with the actions of replenishing qi and reinforce the healthy qi,can regulate the immunologic function of the body through influencing immune organs and controlling the expressions of brain TGF-β1 and TNF-α.