2007•The Chinese Journal of Modern Applied PharmacyRequires access
The Changes of Collagen Metabolism in Rat Heart with Type 2 Diabetes and Preventive Effect of Rosiglitazone
Cui Weib
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Abstract
OBJECTIVE To Investigate the changes of collagen accumulation,the MMP-2,MMP-9 and TIMP-1 mRNA expressions in cardiac tissue of the rat with type 2 diabetes at different stages and the role of rosiglitazone.METHODS The experimental type 2 diabetic rats were induced by injecting streptozotocin(STZ,30mg/kg) and fed with high fat and glucose food.Rats were divided into two groups: 12-week group included control and diabetic rats and 24-week group included control,diabetic rats and rosiglitazone treated diabetic rats.The collagen content,MMP-2,MMP-9 and TIMP-1 mRNA expressions and the protein level of MMP-2,MMP-9,TIMP-1 were determined. RESULTS The collagen content were increased in diabetic rat hearts.The mRNA expression and the protein level of MMP-2 were decreased,the mRNA expression and the protein levels of MMP-9,TIMP-1 were increased,but the ratio of MMP-9/TIMP-1 was decreased in diabetic rat hearts.The collagen accumulation decreased in rosiglitazone-treated rat hearts.Rosiglitazone increased MMP-2 mRNA expression and the level of protein.Meanwhile,MMP-9 and TIMP-1 mRNA and protein were reduced,but the ratio of MMP-9/TIMP-1 increased in rosiglitazone-treated rat hearts.CONCLUSION Imbalance of the ratio of MMPs/TIMPs might relate to the pathogenesis of diabetic cardiomyopathy.Rosiglitazone may attenuated the progression of diabetic cardiomyopathy by regulating the mRNA expression and the protein level of MMP-2,MMP-9,TIMP-1.