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2004•Chinese Journal of Spectroscopy LaboratoryRequires access

Spectrophotometric Determination of Cobalt in Biological Sample by Microwave Digestion and Solid Phase Extraction

Bo Yang

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Abstract

Based on the color reaction of 2-(2-quinolinylazo)-1,5-dihydroxy-benzene(QADHB) with cobalt(Ⅱ) and the solid phase extraction of its colored complex with C 18 cartridge, a new method for the determination of cobalt was studied. In the presence of CTMAB and pH 4.0 buffer solution medium, QADHB can react with cobalt(Ⅱ) to form a stable 2∶1 complex. The colored complex was extracted by C 18 cartridge and eluted by ethanol (containing 2% of acetic acid).Then it was determined by spectrophotometry at 580nm.Beer's law is obeyed in the range of 0-0.8μg/mL. This method can be applied to the determination of cobalt in biological samples with satisfactory results.

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What this paper is about

Based on the color reaction of 2-(2-quinolinylazo)-1,5-dihydroxy-benzene(QADHB) with cobalt(Ⅱ) and the solid phase extraction of its colored complex with C 18 cartridge, a new method for the determination of cobalt was studied. In the presence of CTMAB and pH 4.0 buffer solution medium, QADHB can react with cobalt(Ⅱ) to form a stable 2∶1 complex. The colored complex was extracted by C 18 cartridge and eluted by ethanol (containing 2% of acetic acid).Then it was determined by spectrophotometry at 580nm.Beer's law is obeyed in the range of 0-0.8μg/mL. This method can be applied to the determination of cobalt in biological samples with satisfactory results.

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Available abstract

Based on the color reaction of 2-(2-quinolinylazo)-1,5-dihydroxy-benzene(QADHB) with cobalt(Ⅱ) and the solid phase extraction of its colored complex with C 18 cartridge, a new method for the determination of cobalt was studied. In the presence of CTMAB and pH 4.0 buffer solution medium, QADHB can react with cobalt(Ⅱ) to form a stable 2∶1 complex. The colored complex was extracted by C 18 cartridge and eluted by ethanol (containing 2% of acetic acid).Then it was determined by spectrophotometry at 580nm.Beer's law is obeyed in the range of 0-0.8μg/mL. This method can be applied to the determination of cobalt in biological samples with satisfactory results.

Key concepts: Cobalt, Spectrophotometry, Chemistry, Extraction (chemistry), Solid phase extraction, Color reaction, Chromatography, Acetic acid

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