Isolation of canine distemper virus

Canine distemper virus(CDV) was isolated from dead dogs which were suspected as canine distemper through epidemiologic investigation,clinical symptom detection and ELISA diagnosis.The intestinal contents were collected from affected dogs for virus isolation in Vero monkey kidney(Vero)cells and Madin-Darby canine kidney(MDCK)cell culture.The virus RNA was detected by reverse transcription-polymerase chain reaction(RT-PCR).The results showed that the affected material could multiply rapidly in Vero cells,MDCK cells and caused cytopathogenic effect(CPE).A DNA fragment of 760bp was amplified from infected Vero,MDCK cells by RT-PCR.A virus strain was isolated from infected Vero cells and designated as CDV-GZ_1.The other was isolated from infected MDCK cells and designated as CDV-GZ_2.