Studies on the methods of Papaya explant disinfection

The meristem,lateral buds and seedlings of Papaya plants with bisexual or female flowers were taken as the explants for studying the methods of explant disinfection.The best explants for mass propagation were the new lateral buds from the adult trees in the green house.The best method of the surface disinfection was that the explants with the leaves removed,especially the cicatricle,were brushed with alcohol cotton,subsequently immersed in 75% alcohol for 1 minute,followed by disinfected with 1.5% sodium hypochlorite for 10 minutes,and then disinfected with 1% AgNO3 for 10 minutes,finally rinsed with sterile water five times.