GST fusion expression of the mature peptide of human protein S in E.coli

Objective To construct a prokaryotic vector for the expression of human protein S. Methods The coding sequence of protein S mature peptide was amplified from an existing recombinant plasmid (PS pcDNA3) with primers designed in our laboratory, and cloned into pGEX-2T after restrictive digestion with EcoRI and BamHI . GST-protein S fusion protein was expressed after induction by IPTG. Results Sequencing and restriction digestion of the recombinant plasmid revealed the existence of the coding sequence for protein S mature peptide. SDS-PAGE showed that a protein band of about 96 kD could be induced by IPTG in the recombinant bacteria harboring the plasmid. Conclusion The coding sequence of protein S mature peptide is introduced into the pGEX-2T plasmid and a GST-fused protein S mature peptide could be induced at a high level.