New microtubule inhibitor of YB-B1S-induced apoptosis in Hela cells and its mechanism

Objective:To investigate whether the derivative of indol-3-glyoxylic acid amide induced apoptosis on human galactophore cancer Hela cells line(Hela cells)in vitro and the mechanism.Methods:MTT assay,cell growth curve,flow cytometry(FCM),fluorescence microscopy,DNA ladder,RT-PCR,were carried out,and the changes of cytoskeleton was detected by confocal microscopy.Results:The cytotoxic effecet on Hela cells could be observed by MTT assay and cell growth curve,and this effect was in a dose-and time-dependent manner.After treatment of Hela cells with YB-B1S,typical apoptotic features were observed under the fluorescent microscopes by the Hoechst staining.We also found the typical sub-G1 peak and cells blocked in G2+M phase by flow cytometry.RT-PCR showed that YB-B1S significantly up-regulated caspase-3 and down-regulated Bcl-2 mRNA expressions in Hela cells.The disrupted cytoskeleton can be found by the confocal microscopy.Conclusion:YB-B1S showed obvious anticancer activity in vitro by inducing apoptosis and causing cell cycle arrest in the dose-dependent manner and can interfere with microtubule polymerization and disrupting cytoskeleton.