Study on WZSP Semen Cryopreservation Techniques

Frozen boar semen pellets were made on the fluorin plant.To select out a best freezing extender from several extenders and freezing-thawing protocols,sperm viability,motility and plasma membrane integrity were studied in this experiment.The results showed that①the number Ⅳ extender was the best among the extenders tested and its sperm motility(0.427±0.025),viability(0.610±0.036) and plasma membrane integrity(0.503±0.015) after-thawing were all significantly higher than those obtained with number Ⅰ、Ⅱ and Ⅲ extenders(P0.05);② The sperm motility and plasma membrane integrity of semen cooling for 2 h at 4℃ after-thawing were both better than those cooling for 3 h at 17℃ and then transferring in 4℃ refrigerator for 2 h.And the sperm viability had significant differences(P0.05);③The effects of thawing in solution were better than those out of dried thawing.