Determination of Ganoderma acid A in Rongbao Ganoderma No.1 by High Performance Liquid Chromatography

Objective To establish a high performance liquid chromatography(HPLC) method for determining the ganoderma acid A content in the Rongbao Ganoderma lucidum No.1 and its spore powder.Methods The HPLC method was established with UltimateTM XB-C18 column,and the column temperature was 25℃;Flow rate was 0.800 mL/min;Measurement wavelength was 254 nm;and the moving phase was acetonitrile(B) and 0.1% glacial acetic acid-water(A) with gradient elute.Results Ganoderma acid A linear range was from 0.010 mg/mL to 1.200 mg/mL(r=0.9998).The average recovery was 100.55%,and the inner-day RSD was 1.70%(n=6).The ganoderma acid A content range in fruiting bodies was from 2.2217 mg/g to 3.5311 mg/g,and in spore powder was from 0.1672 mg/g to 0.7429 mg/g.Conclusions The method is simple,specific and reproducible.The ganoderma acid A content in the Rongbao Ganoderma lucidum No.1 and its spore powder collected for three consecutive years has been measured,and the results of its content are increased with the increase of the year.