Effect of silence S100A4 by RNA interference on proliferation,apoptosis,chemosensitivity of gemcitabine of the pancreatic cancer cell lines

OBJECTIVE: To observe the effect on the proliferation,apoptosis and chemosensitivity of gemcitabine after slience S100A4 by RNA interference.METHODS: the S100A4-specified small interference RNA was designed and synthesized.The expression of S100A4 was detected by RT-PCR 24 h after the S100A4 siRNA transfected into BxPC-3 and AsPC-1 cells.The growth curve of BxPC-3 and AsPC-1 cells were recorded to demonstrate the proliferation.The apoptosis of BxPC-3 and AsPC-1 cells was tested by flow cytometric analysis.The gemcitabine of IC50 was measured by MTT.RESULTS: S100A4 expression was suppressed significantly after S100A4 siRNA transfected into BxPC-3 and AsPC-1 cells.The abilities of proliferation were inhibited significantly after S100A4 siRNA transfected.TUNEL assay showed apoptosis increased after transfection.Before and after slience S100A4 by siRNA,IC50 of gemcitabine in control was(9.95±0.34) mmol/L,Negative Control was(9.641±0.434) mmol/L,Interference was(1.182±0.175) μmol/L,respectively;in AsPC-1,control was(64.15±3.41) mmol/L,Negative Control was(65.19±4.4) mmol/L,Interference was(1.962±0.113) mmol/L,respectively.CONCLUSIONS: S100A4 slience can inhibite cell proliferation and enhence the apoptosis as well as increase the chemosensitivity of gemcitabine.S100A4 could serve as a potential target in the filed of pancreatic cancer therapy.