ScholarLens Back to search
2002•Journal of LeukemiaRequires access

IN VITRO STUDY ON THE RELATIONSHIP BETWEEN NF-κB AND THE APOPTOSIS EFFECT OF DAUNORUBICIN (DNR) ON HL-60 CELLS

Huang Zong-ga

Open publisher page 0 citations

Abstract

Objective:To study whether NF-κB has the relationship with the apoptosis effect of daunorubicin (DNR) on HL-60 cells in vitro.Methods:This study observed the apoptosis of HL-60 by optic microscope,flow cytometry (FCM) and detected expression or activation of NF-κB with immunocytochemistry (IC) and flow cytometry (FCM) or EMSA of the groups:control HL-60 cells,HL-60 cells treated with DNR,HL-60 cells treated PDTC or FBI before DNR.Results:When treated with 1.0 μmol/L DNR,there showed the typical apoptotic cells.It was shown that after HL-60 cells were treated with 1.0 μmol/L DNR,the expression of NF-κB increased markedly compared with the control HL-60 cells.When adding PDTC,this effect of DNR was decreased,but FBI hadn′t presented these activation.Conclusion:It is suggested from the results that NF-κB has something to do with this apoptosis process and ROS had the marked correlation with the apoptosis process.

About this research paper

What this paper is about

Objective:To study whether NF-κB has the relationship with the apoptosis effect of daunorubicin (DNR) on HL-60 cells in vitro.Methods:This study observed the apoptosis of HL-60 by optic microscope,flow cytometry (FCM) and detected expression or activation of NF-κB with immunocytochemistry (IC) and flow cytometry (FCM) or EMSA of the groups:control HL-60 cells,HL-60 cells treated with DNR,HL-60 cells treated PDTC or FBI before DNR.Results:When treated with 1.0 μmol/L DNR,there showed the typical apoptotic cells.It was shown that after HL-60 cells were treated with 1.0 μmol/L DNR,the expression of NF-κB increased markedly compared with the control HL-60 cells.When adding PDTC,this effect of DNR was decreased,but FBI hadn′t presented these activation.Conclusion:It is suggested from the results that NF-κB has something to do with this apoptosis process and ROS had the marked correlation with the apoptosis process.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective:To study whether NF-κB has the relationship with the apoptosis effect of daunorubicin (DNR) on HL-60 cells in vitro.Methods:This study observed the apoptosis of HL-60 by optic microscope,flow cytometry (FCM) and detected expression or activation of NF-κB with immunocytochemistry (IC) and flow cytometry (FCM) or EMSA of the groups:control HL-60 cells,HL-60 cells treated with DNR,HL-60 cells treated PDTC or FBI before DNR.Results:When treated with 1.0 μmol/L DNR,there showed the typical apoptotic cells.It was shown that after HL-60 cells were treated with 1.0 μmol/L DNR,the expression of NF-κB increased markedly compared with the control HL-60 cells.When adding PDTC,this effect of DNR was decreased,but FBI hadn′t presented these activation.Conclusion:It is suggested from the results that NF-κB has something to do with this apoptosis process and ROS had the marked correlation with the apoptosis process.

Key concepts: Apoptosis, Daunorubicin, Flow cytometry, In vitro, Molecular biology, Immunocytochemistry, NF-κB, Chemistry

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
IN VITRO STUDY ON THE RELATIONSHIP BETWEEN NF-κB AND THE APOPTOSIS EFFECT OF DAUNORUBICIN (DNR) ON HL-60 CELLS — Research Paper | ScholarLens