The research of Pim-3 protective effect on cardiomyocyte anoxia-reoxygenation injury

Aim To study the protective effect of proto-oncogene Pim-3 on cardiomyocytes aganist anoxia-reoxygenation(A/R)injury.Methods The A/R and anoxia preconditioning(APC)models of primary cultured neonatal rat myocytes were established and the expression vector pEGFP-N2/Pim-3 inserted with wild type target gene Pim-3 was transfected into the cardiomyocytes.Expression of Pim-3 was detected by RT-PCR and Western blot.LDH activity,MTT and TUNEL were detected after treatment.Results Pim-3 showed little expression in the control group but did high expression in A/R injury,Furthermore,pim-3 showed a higher expression in the APC+A/R group than in the A/R group.The efficiency of transfection of exogenous genes into cardiomyocytes was as high as 30% determined by fluorescence microscope after 24 h.In the pEGFP-N2/Pim-3+A/R group,the cellular survival was significantly increased while the LDH activity and the apoptotic index of cell were markedly decreased compared with the A/R group.Conclusion Pim-3 should be involved in the role of APC protection and transfecting of exogenous Pim-3 into cardiomyocytes could protect cardiomyocytes against the A/R injury.