STUDY ON DETECTION OF INFLUENZA TYPE A H1N1 VIRUS BY REAL-TIME RT-PCR ASSAY

[Objective]To establish a specific,sensitive and rapid method of real time RT-PCR assay for the detection of influenza type A H1N1 virus RNA.[Methods]The specific primers and probes were recommend by WHO The primers and probes as well as the reaction condition were optimized to improve the sensitivity and specificity of the assay.The throat swab specimens which were suspected of carrying in influenza Type A H1N1 virus were detected by using the method.[Results]The sensitivity of this assay was 10-7(the titer of HA is 1︰128),and the viral RNA could be detected directly from the clinical specimens.It took only 2.5 hours to complete the whole course of reaction including extraction of viral RNA and the real-time RT-PCR.[Conclusion]It concludes that the TaqMan-based real-time RT-PCR assay is a rapid,sensitive,specific and new method for the detection of influenza Type A H1N1 virus,which could be used as an emergency detection by the basic CDC and hospital.