Effect of mild hypothermia at focal side on neuron surrivial in rats after focal cerebral ischemia and reperfusion

Objective To explore the therapeutic effectiveness of local mild hypothermia on expression of Survivin and NF-κB to study the relation of apoptosis-inhibiting protein Survivin in rats after focal cerebral ischemia and reperfusion injury.Methods The focal cerebral ischemia and reperfusion model of rats was achieved by intraluminal middle cerebral artery occlusion (MCAO).The adult rats were randomly divided into sham-operation group,normothermia ischemia and reperfusion group and hypothermia ischemia and reperfusion group.The ischemic groups were respectively further divided into Subject ischemia for 2h,3h,6h,8h respectively and then all reperfused for 4h,24h,72h,1w,2w,4 rats per time point.Brain mild hypothermia was achieved 13~14 minutes after ischemia and reperfusion for 4h.The expression of Survivin and NF-κBP65 were assayed by immunohistochemistry.Results Compared with the normothermia group,focal mild hypothermia greatly increased the expression of Survivin and obviously decreased the expression of NF-κBP65 in penumbra(P0.05orP0.01).Conclusion The focal mild hypothermia can accelerate the recovery of the neurological outcome.The neuroprotection of mild hypothermia may be related to the inhibition of the activation of NF-κB P65,enhancing Survivin expression in penumbra.