The expression of cyclin D1,CDK4,p16, Rb and their significance in benign and malignant lesions of the pancreas

Objectives[WT5”BZ] To investigate the expression of cyclin D1,CDK4, p16,Rb and their significance in benign and malignant lesions of the pancreas.[WT5”HZ] Method[WT5”BZ] Avidin-biotin complex method was used on paraffin-embedded section to investigate tissues of pancreatic carcinoma in 53 cases and 17 cases with pancreatitis.[WT5”HZ] Results[WT5”BZ] The positive rates of cyclin D1,CDK4 and Rb were significantly higher in cases with pancreatic carcinoma than that with chronic pancreatitis and paracancerous epithelium, the expression of p16 was lower in the former than that in the later. Most of the positive cases of cyclin D1, CDK4, Rb and the negative ones of p16 were mildly or severely atypical hyperplasia of pancreatic epithelium in chronic pancreatitis and paracancerous epithelium. The positive rates of cyclin D1, CDK4 and Rb in the pancreatic carcinoma of well-differentiation and without metastasis[CM(43] were significantly lower than that in the ones of poor-differentiation and with metastasis, and that of p16 was the opposite. The positive correlation was found between the expressison of cyclin D1 and CDK4, the negative correlation was also found between the expressison of p16 and Rb in pancreatic carcinoma.[WT5”HZ] Conclusions[WT5”BZ] The expressions of cyclin D1, CDK4, p16 and Rb might be related to the carcinogesis, development, biological behaviors and prognosis of pancreatic carcinoma. Abnormality in the negative feedback regulatory pathway of cyclin D1/CDK4, Rb and p16 might be involved in the molecular mechanism of pancreatic carcinoma. [WT5”HZ]