Spectrophotometric study on the antioxidant activity of chinese aloe leaf
Xin-Hong Wang
Abstract
Xin-Hong Wang
Abstract
Chinese aloe leaf has been chosen to evaluate its antioxidant of radicals ·OH and ·DPPH by using their extracts from different kinds of solvents and determining whether there is a correlation between the active component and capabilities.Their antioxidant capabilities were evaluated quantitatively with IC50.The extract from ethyl acetate has a higher scavening rate on ·OH and DPPH radical,its IC50 is 4.41mg·mL-1 dw,5.73mg·mL-1 dw,respectively.Phenolic compounds and total flavonoid content from extracts were also been measured in this experiment;total antioxidant capacity has been studied through FRAP assay contrasting to standard antioxidants.A significant correlation was established between the total phenolic content and total flavonoid content,which imply phenolic compounds is main antioxidation in Chinese aloe leaf.
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Chinese aloe leaf has been chosen to evaluate its antioxidant of radicals ·OH and ·DPPH by using their extracts from different kinds of solvents and determining whether there is a correlation between the active component and capabilities.Their antioxidant capabilities were evaluated quantitatively with IC50.The extract from ethyl acetate has a higher scavening rate on ·OH and DPPH radical,its IC50 is 4.41mg·mL-1 dw,5.73mg·mL-1 dw,respectively.Phenolic compounds and total flavonoid content from extracts were also been measured in this experiment;total antioxidant capacity has been studied through FRAP assay contrasting to standard antioxidants.A significant correlation was established between the total phenolic content and total flavonoid content,which imply phenolic compounds is main antioxidation in Chinese aloe leaf.
Key concepts: DPPH, Antioxidant, Chemistry, Flavonoid, Ethyl acetate, Antioxidant capacity, Food science, IC50